Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 10;4(5):ENEURO.0332-16.2017. doi: 10.1523/ENEURO.0332-16.2017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Allen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Figure 6. — Mutation strategy to restrict exon choice at e18a locus of mouse Cacna1b gene. A, Schematic illustrates homologous recombination strategy used to generate two mouse strains that express either +18a-only (Cacna1b^tm3.1Dili) or Δ18a-only (Cacna1b^tm4.1Dili) Ca_V2.2 transcripts. Targeting constructs contained 4.4 kb (left) and 2.3 Kb (right) recombination arms, and a NeoR cassette was used to select for successful recombination events in embryonic stem cells. B, left, Splicing patterns in Cacna1b pre-mRNA of WT, +18a-only, and Δ18a-only mice. Right, RT-PCR products from WT, +18a-only Ca_V2.2, and Δ18a-only Ca_V2.2 mouse brain. Primers were located in e18 and e19 as described in Figure 1. Expected bands were observed: two different size PCR products corresponding to +18a and Δ18a from WT, one band corresponding to +18a from +18a-only samples, and one band corresponding to Δ18a from Δ18a-only samples.