Table 1. Representative yields of EGFP-39-TAG-151-TGA reporter incorporating various combinations of ncAAs.
ncAA at 39TAG | ncAA at 151TGA | Yield (μg/10 cm dish) | Yield (% of wild-type reporter) |
Tyr (wild type) | Tyr (wild type) | 175 | |
BocK, 6 | OMeY, 1 | 13 | 7.5 |
hPrK, 8 | AzF, 2 | 2 | 1.1 |
PrY, 3 | AzK, 7 | 1.1 | 0.6 |
AzK, 7 | AcF, 4 | 1.8 | 1 |
CpK, 9 | AzF, 2 | 8 | 4.6 |
Cap, 5 | BocK, 6 | 4.5 | 2.6 |