Figure 3.

Effect of PNA-Loaded NPs In Vivo

(A) miR-210 overexpression in isolated HeLa tumor cells and in the bulk tumors from xenograft mouse models compared to HeLa cells incubated for 24 or 48 hr in 0.5% hypoxia (n = 3, data represented as mean ± SE). t test was used for statistical analysis relative to normoxic HeLa cells. (B) Fold-change in tumor growth in response to locally administered anti-miRs. Arrowhead represents 6-mg nanoparticle injection (n = 5 for each group, data represented as mean ± SEM). ANOVA was used for statistical analysis for each group relative to the blank group. (C) Number of days for tumors to reach 3x their initial volume at the time of treatment (n = 5 for each group, data represented as mean ± SEM). Long-rank test was used for statistical analysis for each group relative to the blank group. (D) Representative tumor images after intratumoral treatment of nanoparticles. In accordance with animal care regulations, mice were euthanized after tumors reached a volume of 1,000 mm³ for the control blank, mismatched γPNA, and regular PNA-treated group. Because the gamma PNA-treated mice showed a delayed tumor growth, they were maintained for a longer period and euthanized 46 days after treatment. The tumor images were taken before the mice were euthanized. (E) Relative miR-210 levels in RNA extracted from HeLa tumor cells derived from xenograft tumors treated with the indicated nanoparticles (n = 3, data represented as mean ± SE). t test was used for statistical analysis, p < 0.05. (F) Western blot of protein extracted from tumors treated with the indicated nanoparticles.