Figure 1.

A8301 Inhibits Smad2 Phosphorylation In Vitro and In Vivo

(A) Cultured M3-9-M and 76-9 cells were exposed to 1 ng/mL recombinant TGF-β for 30 min alone or in the presence of 1 μM A8301. FACS analysis for TGF-βR1 expression was performed with unstimulated cells. (B) Immunocompetent C57BL/6 mice bearing M3-9-M or 76-9 tumors were given intratumoral injections of vehicle control (DMSO in PBS) or 6 mg/kg A8301 every other day for a total of three doses and then sacrificed 24 hr after the final dose.