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. 2017 Sep 22;14:229–236. doi: 10.1016/j.redox.2017.09.016

Fig. 2.

Fig. 2

Sirt1 regulates Sirt3 expression through AMPK-PGC1 pathway after OGD. Cells were transfected with siRNA for 48 h or pretreated with salermide (50 μM) for 30 min, and exposed to OGD. The expression of Sirt1 and Sirt3 was detected by western blot (A) and calculated (B and C). Cells were pretreated with salermide (50 μM) or compound C (5 μM) for 30 min and exposed to OGD. The expression of Sirt1 and Sirt3 was detected by western blot (D) and calculated (E and F). Cells were transfected with siRNA for 48 h with or without compound C, or pretreated with salermide (50 μM) for 30 min with or without compound C, and exposed to OGD. The expression of Sirt3 was detected by western blot (G). Cells were transfected with Si-control or Si-PGC1 for 48 h and exposed to OGD. The expression of Sirt3 was detected by western blot (H). Data are shown as mean ± SEM. *p < 0.05 vs. Control. #p < 0.05 vs. OGD. &p < 0.05 vs. Si-control. $p < 0.05.