Fig. 5.

Effects of Sirt1-Sirt3 axis on ROS generation. Cells were treated with salermide (50 μM) or compound C (5 μM) and exposed to OGD. Intracellular ROS generation was detected by DHE probe (A) and calculated (B). Cells were transfected with siRNA for 48 h and exposed to OGD. ROS levels were measured (C). Mitochondrial superoxide was measured by MitoSOX up to 60 min after OGD (D). Cells were treated with DPPH (50 μM) with different concentrations of salermide or BHA (5, 10, 50 or 100 μM), and radical scavenging activity was determined (E). Cells were treated with Antimycin with or without salermide or FeTCCP, and mitochondrial superoxide was determined (F). Cells were treated with salermide or compound C and exposed to OGD. The expression of 3-NT in isolated mitochondria was detected by western blot (G). Scale bar = 20 µm. Data are shown as mean ± SEM. *p < 0.05 vs. Control. #p < 0.05 vs. OGD.