Fig. 1.
Experimental schedule and preventative effect of butaselen against hepatocarcinogenesis. (A) Chemical structure of butaselen (BS). (B) Diethylnitrosamine (DEN), CCl4 and 10% ethanol were used to induce model of hepatocellular carcinoma (HCC) for 24 weeks in C57BL/6J mice. Different doses of butaselen (BS) were administered in other three groups. (C) The body weight change and (D) survival proportions of mice in 180 days. (E) Representative hepatocellular damage at week 6. (F) Gross pathology and HE stain of cirrhosis at week 16. (G) Gross and pathological change of HCC at week 24. Data were shown as mean ± std, n ≥ 28 and analyzed with ANOVA or Log-rank test. *, #p < 0.05 compared with control or model group. BSL, 9 mg/kg BS; BSM, 18 mg/kg BS; BSH, 36 mg/kg BS.