Table 2.
Engraftment in NSG Mice
| Mouse No. | Control |
sCD4 |
||
|---|---|---|---|---|
| CD45+ Cells (%) | ZsGreen1+ Cells (%) | CD45+ Cells (%) | ZsGreen1+ Cells (%) | |
| 1 | 62 | 25 | 48 | 4 |
| 2 | 8 | 53 | 55 | 5 |
| 3 | 20 | 34 | 47 | 14 |
| 4 | 2 | 7 | 47 | 6 |
| 5 | 6 | 73 | 22 | 25 |
| 6 | 23 | 28 | 31 | 18 |
| 7 | 42 | 10 | 2 | 12 |
| 8 | 14 | 9 | 25 | 43 |
| 9 | 80 | 5 | 21 | 5 |
| 10 | – | – | 54 | 4 |
| 11 | – | – | 46 | 2 |
| 12 | – | – | 11 | 1 |
| Arithmetic mean | 29 | 27 | 34 | 12 |
| Geometric mean | 17 | 19 | 26 | 7 |
| Median | 20 | 25 | 39 | 6 |
Mice were injected with HSPCs transduced with LV-EF1α (control) or LV-EF1α-AAT-sCD4 (sCD4). The peripheral blood of mice was analyzed for the presence of human CD45+ cells and for the percentage of ZsGreen1+ cells within the CD45+ cell population 13–19 weeks post-injection of HSPCs.