Table 3.
Inhibition constants (Ki) and modes of inhibition.
| Isoflavone | Metabolic activity | Ki (μM) | Mode of inhibition |
|---|---|---|---|
| Genistein | E1 sulfation | 0.76 | Non-competitive |
| E2 sulfation | 0.32 | Non-competitive | |
| E2 glucuronidation | 6.01 | Non-competitive | |
| Daidzein | E1 sulfation | 1.64 | Non-competitive |
| E2 sulfation | 0.48 | Non-competitive | |
| E2 glucuronidation | 7.31 | Non-competitive | |
Ki values were obtained by plotting the slopes of the respective Lineweaver–Burk plots against isoflavone concentration. All data represent the means ± SD of four independent biological replicates.