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. 2017 Oct 10;13:298. doi: 10.1186/s12917-017-1218-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Analysis of curcumin treatment effect on PRRSV internalization in Marc-145 cells. Pre-chilled Marc-145 cells were infected with GD-HD (MOI: 0.1) at 4 °C for 1 h then incubated with indicated curcumin concentrations at 37 °C for 1 h. After incubation, non-internalized viruses were removed and maintained in medium without curcumin for 36 h. Cells and culture supernatants were harvested to analyze PRRSV N protein expression levels (a) and viral production (b). Each value represents the mean ± SD from three independent experiments (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ND means non-detectable)