Table 1. Primer sets for BERV-K3 and neighboring gene transcripts.

BERV-K3 (P1 in Figure 1B)

For detection of the transcript and preparation of probes for in situ hybridization

P1F: 5′-TCGCCCGAAAGCAGGCTAGTGCTAA-3′

P1R: 5′-CAAGGGCGCAGGCTGTTACCTGTTC-3′

For confirming if BERV-K3 is expressed (in Supplementary Figure S3A)

P1F: 5′-TCGCCCGAAAGCAGGCTAGTGCTAA-3′

P1R′: 5′-GCAAGGTTCCGTTTTATGG-3′

For 5′-RACE primer (in Supplementary Figure S3B)

P1R: 5′-CAAGGGCGCAGGCTGTTACCTGTTC-3′

For isolated predicted full length (in Supplementary Figure S3C)

PFF: 5′-ACGGGTAACAAGGAGTCAAAAG-3′

PFR: 5′-CCCTGATGACAAAGTGACCTCC-3′

The primer set to detect LOC100848658 (P2 in Figure 1B) transcript

F: 5′- GCGTCTACCCCAAAACCAGA-3′

R: 5′- ACAGAGAAAGGTGGTCAGGG-3′

The primer set to detect TCF7 (NM_001099186.2)

F: 5′- CTGTGAGCTGGTTCACCCAT-3′

R: 5′- TCCGCAATGACTTTGGCTCT-3′

The primer set to detect ACTB (NM_173979.3)

F: 5′-CTCTTCCAGCCTTCCTTCCT-3′

R: 5′-GGGCAGTGATCTCTTTCTGC-3′

F, forward primer; R, reverse primer.