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. 2017 Sep 23;73(Pt 10):550–554. doi: 10.1107/S2053230X17012894

Table 1. Crystallization information.

Method Hanging-drop vapour diffusion
Plate type 24-well plates
Temperature (K) 293
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM phosphate buffer pH 7.0
Composition of reservoir solution 2.0–2.3 M ammonium sulfate, 1.7%(w/v) PEG 400, 15%(v/v) glycerol, 85 mM HEPES pH 7.5
Volume and ratio of drop 4 µl (1:1 protein:reservoir solution)
Volume of reservoir (ml) 0.45