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Expression of the putative EPPase in nondiapaused eggs. Diapaused eggs and nondiapaused eggs were collected, and total RNA was isolated. The RNA was isolated from nondiapaused eggs at the late blastula stage, which is when cell division stops. After reverse transcription of the RNA to cDNA, the EPPase was quantified by RT-PCR and normalized to the expression level of the ribosomal protein L32.
