Figure 3.

Arteries from mice deficient in PEA‐15 (phosphoprotein enriched in astrocytes 15; PEA‐15 ^−/−) undergoing neointimal hyperplasia have increased proliferation markers and decreased smooth muscle differentiation markers. The expression of the proliferation marker PCNA (proliferating cell nuclear antigen) and the smooth muscle differentiation marker smoothelin were determined in carotid arteries from PEA‐15 ^−/− and wild‐type (WT) mice following vascular injury and compared with the contralateral uninjured artery or arteries from sham‐operated mice. Protein expression was assessed at 3 time points after injury: (A) 3 days, (B) 7 days, and (C) 14 days. Representative immunoblots are shown for each time point, and mean data are expressed as a ratio of GAPDH protein expression, n=3 for each treatment. Statistical comparisons are shown only for sham vs injured arteries. *P<0.05 and **P<0.01 using 2‐way ANOVA and Bonferroni post hoc test for all graphs.