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. 2017 Sep 11;6(9):e006936. doi: 10.1161/JAHA.117.006936

Figure 4.

Figure 4

Collagen content is decreased and smooth muscle cell number is increased in arteries from mice deficient in PEA‐15 (phosphoprotein enriched in astrocytes 15; PEA‐15 −/−) undergoing neointimal hyperplasia. A, The collagen content was determined in PEA‐15 −/− and wild‐type (WT) mice by analyzing sections of arteries stained with Masson's trichrome (representative images are shown). Arteries are compared from mice at 3, 7, and 14 days after injury. Collagen is shown as a percentage of area of the total arterial wall, n=6 for each treatment. *P<0.05 and **P<0.01 using 1‐way ANOVA and Newman–Keuls post hoc test. B, Smooth muscle cell content was assessed by counting the number of cells stained positive for anti–smooth muscle α‐actin antibody in the neointima only (not media) at each time point. This was compared in PEA‐15 −/− and WT mice at 3, 7, and 14 days after injury, n=4 for each treatment. For clarity, only relevant significant comparisons are shown. *P<0.05 using 1‐way ANOVA and Newman–Keuls post hoc test.