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. 2017 Aug 2;37(4):BSR20170976. doi: 10.1042/BSR20170976

Figure 3. Occupancy of transcription factors in binding motif mutant clones.

Figure 3

Control cells and clones with GATA-1 (A), TAL1 (B), or KLF1 (C) motif deletion were subjected to ChIP using antibodies specific for GATA-1, TAL1, or KLF1. Amounts of immunoprecipitated DNA were compared with input DNA and then normalized compared with amounts of immunoprecipitated DNA in the mouse β-globin LCR HS2. The mouse Actin gene served as an internal negative control and normal goat IgG (IgG) as an experimental negative control. Results are the means ± S.E.M. of two independent experiments; **P<0.01.