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(A)PCR products amplified by A1F/R, A2F/R and A3F/R primers were visualized on agarose gels in HS3ΔGA and HS3ΔTA clones. M is the DNA marker. Dissociation curves generated by qPCR were presented for A1F/R and A2F/R primersets. (B) Dissociation curves of qPCR were presented for A1F/R and A2F/R-1 primer sets inHS3ΔKL clones.
