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Characteristics of the primers used in real-time RT-PCR
| Gene | ||
|---|---|---|
| VEGF | 18s RNA | |
| NCBI number | NM_001025370 | X03205 |
| Initiator F | AAGGAGGAGGGCAGAATCAT | GTAACCCGTTGAACCCCATT |
| Primer length | 20 | 20 |
| Initiator R | ATCTGCATGGTGATG TTGGA | CCATCCAATCGGTAGTAGCG |
| Primer length | 20 | 20 |
| Segment length | 226 | 152 |
| Annealing temperature | 60 ℃ | 56.5℃ |
