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. 2017 Oct 4;12:7255–7272. doi: 10.2147/IJN.S136726

Figure 1.

Figure 1

Synthesis of cRGD-siRNA conjugates.

Notes: Conditions and reagents: i–iii – Applied Biosystems 394 DNA/RNA synthesizer; iv – methylamine alcohol solution, concentrated ammonia water, 60°C, 90 min; TEA⋅3HF, 65°C, 90 min; anion-exchange HPLC; v – 0.1 M DTT in 0.02 M Tris-HCl (pH 8.3–8.5), room temperature, 3 h; vi – PBS buffer including 10 mM EDTA, pH 7.2, Ar, room temperature, 12 h; vii – PBS buffer, pH 7.2, room temperature, 3 h; viii – Annealing at 85°C, GeneAinp PCR System 2400.

Abbreviations: cRGD, cyclo(Arg-Gly-Asp-d-Phe-Lys[PEG-MAL]) peptide; siRNA, small interfering RNA; TEA, triethylamine; Tris, tris(hydroxymethyl)aminomethane; PBS, phosphate-buffered saline; EDTA, ethylenediaminetetraacetic acid; PCR, polymerase chain reaction.