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. 2017 Oct 9;7:12867. doi: 10.1038/s41598-017-12740-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of founder mouse #3. (A) Fibre-FISH showing the presence of a wild type (WT), inversion (INV) and duplication (DUP2) allele in the founder. (B) Schematic of the INV allele detected in the founder as well as in one F1 offspring. The FW junction has a 62 bp insertion of the bridge oligo (blue). The RV junction for this allele did not yield a PCR product in the offspring. (C) Schematic of the second inversion allele with the deletion junction being present within the FW PCR fragment. Sequence of the FW PCR in the founder as well as three offspring revealed that the R2 primer (purple arrowhead) was present within the FW PCR fragment. Sequence of the RV PCR identified a 892 bp deletion removing both the R1 and R2 primer binding sites.