Fig. 5.

Sex hormone treatment protects against gonadectomy-induced trabecular bone loss in the 5th lumbar vertebrae of female and male Dmp1Cre.Socs3 ^f/f and Dmp1Cre mice. a Schematic showing experimental protocol including sham operation, ovariectomy (OVX), or orchiectomy (ORX) at 6 weeks of age, and treatment with silastic implants of dihydrotestosterone (DHT) or estradiol (E₂) from 6–12 weeks of age. b–g Micro-CT analysis including representative transaxial images of the 5th lumbar vertebrae of female Dmp1Cre (b), Dmp1Cre.Socs3 ^f/f (c), and male Dmp1Cre (e), Dmp1Cre.Socs3 ^f/f (f) mice from each treatment group and vertebral trabecular bone volume of female (d) and male (g) Dmp1Cre and Dmp1Cre.Socs3 ^f/f mice subjected to gonadectomy and steroid treatments as outlined in a. Values are mean + SEM, n = 6 per group. *p < 0.05; **p < 0.01; ***, p<0.001 for comparisons indicated in the figure