Fig. 6.

Androgen treatment rescues the female Dmp1Cre.Socs3 ^f/f defect in corticalization, whereas estradiol treatment of male Dmp1Cre.Socs3 ^f/f mice recapitulates the female phenotype. Treatment protocol is shown in Fig. 5a. a, b, e, g, i, k Female mice. c, d, f, h, j Male mice. Shown are transaxial cross sections at the top of the femoral metaphyseal region of analysis in female (a, b) and male (c, d) Dmp1Cre (a, c), and Dmp1Cre.Socs3 ^f/f (b, d) mice sham-operated or gonadectomized (ovariectomy OVX, orchiectomy ORX) and treated with silastic implants of dihydrotestosterone (DHT) or estradiol (E2) from 6–12 weeks of age. Data shown are cortical thickness, including both compact cortical bone, and porous (spongy) bone, the latter shown in hatched bars (e, f), and cortical porosity measured at both the upper (g, h) and lower (i, j) metaphysis in female (e, g, i) and male (f, h, j) Dmp1Cre and Dmp1Cre.Socs3 ^f/f mice sham-operated or gonadectomized and treated with silastic implants of DHT or E₂ from 6–12 weeks of age. Values are mean + SEM, n is shown for each group in e, f; *p < 0.05; **p < 0.01 for comparisons indicated in the figure by two-way ANOVA with Sidak post-hoc test. k, l Representative histological images of calcein labels in the corticalization zone on the lateral side of the tibial metaphysis for each treatment group. Orange = bone; green = calcein; m = marrow; b = bone