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. 2017 Oct 9;7:12821. doi: 10.1038/s41598-017-13029-4

Figure 1.

Figure 1

Renal non-heme iron levels are increased in the NZB/W model of SLE. NZB/W mice underwent weekly testing for albuminuria (defined as >2+ or >100 mg/dL in 24 h urine samples) and were sacrificed at the onset of albuminuria (32–36 weeks). Tissues and plasma from these mice were compared with age-matched NZW mice and 8 and 20 week old NZW and NZB/W mice. Non-heme iron concentration expressed per g wet tissue weight in (A) renal cortex and (B) outer medulla. (C) Anti-ds DNA IgG was measured in plasma. Mice were housed in metabolic cages for 24 h to allow sample collection for data depicted in (D) and (E). Urinary transferrin excretion for age-matched NZW and NZB/W mice (n = 4–6 per group) is presented in (D). Data other than in (E) are presented as mean ± SEM for n = 4–6 mice per group, with P values derived by two-way ANOVA, testing for main effects of mouse strain (P Strain), age (P Age) and whether concentrations changed with age differentially between strains (P S*A). *P < 0.05 compared to age-matched NZW mice. (E) Depicts urinary ferritin excretion in individual 32–36 week old NZW and NZB/W mice; data were compared by unpaired Student’s t-test.