Figure 5.

(A) Effect of various mutations on DJ-1 degradation of methylglyoxal-adducted CoA. The Abs₂₈₈ profiles of CoA-derived hemithioacetal in the presence of various DJ-1 mutants over time are shown. The blue, green, and red lines correspond to WT, the C106S mutant, and pathogenic-based mutants, respectively. The Abs₂₈₈ of the reaction lacking DJ-1 was subtracted as a blank control. (B) Degree of hemithioacetal consumption. Values are based on the linear portion of the degradation curves shown in (A) relative to WT DJ-1, which is defined as 100% consumption. Bars represent the mean ± SD (error bars) with statistical significance evaluated using one-way-ANOVA and Dunnett’s test.