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. 2017 Oct 9;7:12842. doi: 10.1038/s41598-017-13103-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and localization of UPIb-EGFP in non-polarized UCs. Immunofluorescence labelling of (A) GM130 (cis-GA marker); (B) giantin (cis- and medial GA marker) and (C) golgin-97 (trans-GA network marker) in UPIb-EGFP expressing UCs shows the presence of UPs in different parts of the GA. (A–C) Arrows show the green signals (UPIb-EGFP), red signals (GM130, giantin, golgin 97) and overlap of green and red signals (merged). Areas in larger frames are 50% increased smaller frames. Bars: 10 µm (A–C).