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. 2017 Oct 9;7:12842. doi: 10.1038/s41598-017-13103-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and localization of UPIb-EGFP and UPIb/UPIIIa-EGFP in non-polarized MDCK cells. MDCK cells were transfected with (A) UPIb-EGFP or (B) co-transfected with UPIb/UPIIIa-EGFP and labelled with the antibody against giantin. (A,B) Both, UPIb-EGFP and UPIb/UPIIIa-EGFP signals overlap with the GA marker giantin (arrows). Smaller frames are 100% enlarged insets on the right. (C,D) The presence of UPIb-EGFP (black dots in C) and UPIb/UPIIIa-EGFP (black dots in D) in the GA is seen also at the ultrastructural level. Bars: 10 µm (A,B); 500 nm (C); 1 µm (D).