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. 2017 Oct 9;7:12842. doi: 10.1038/s41598-017-13103-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of UPIb/UPIIIa-EGFP signal and COPI, COPII or clathrin signal in MDCK cells. After synchronization of protein released from GA, MDCK cells were treated with tannic acid to prevent fusion of UPIb/UPIIIa-EGFP positive vesicles with the plasma membrane. (A–C) Representative images of the localization of UPIb/UPIIIa-EGFP (green) and other vesicle markers (red), as indicated. (D) Quantitative analyses of the localization of UPIb/UPIIIa-EGFP (green) and other vesicle markers show moderate co-localization of UPIb/UPIIIa-EGFP and COPI, COPII or clathrin. The degree of co-localization from the Pearson’s value correlation coefficients was categorized according to⁶⁹ as very strong (0.85 to 1.0), strong (0.49 to 0.84), moderate (0.1 to 0.48), weak (−0.26 to 0.09), and very weak (−1 to −0.27). Error bars are the standard errors of the means (n = 3). Bars: 10 µm (A–C).