Fig 1 Supplement. Conversion of Q1-CX3CL1 to pE1-CX3CL1 in human serum.

At time point 0 min, the major peak corresponds to a mass of 8630, which is close to the theoretical molecular weight (8639) of Q1-CX3CL1. After incubating Q1-CX3CL1 with human serum for 30 min, the major peak corresponds to a mass of 8613, which is close to the theoretical molecular weight (8622) of pE1-CX3CL1. The doublet at 8286/8303 corresponds to a shortened peptide produced by limited proteolysis at the C-terminus (release of Cterminal RNG tripeptide). The C-terminal cleavage is independent of N-terminal cyclisation reaction.

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