Figure 4. NF-κB-dependent co-regulation of QPCT and substrates CCL2 and CX3CL1 mRNA expression in HUVECs.

After treatment with the appropriate inhibitor IKK2 or U0126 (each 10 µM) for 30 min, HUVECs were cultured for 6 h with cytokines TNF-α/IL-1β (100 ng/ml). Results of qPCR are shown relative to the basal control levels (A) QPCT, QPCTL; (B) CX3CL1, CCL2, ICAM1/CD54. Data from three independent experiments are shown as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001).