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. 2017 Aug 24;37(4):BSR20170712. doi: 10.1042/BSR20170712

Figure 5. Transfection of HUVECs with QPCT siRNA resulted in down-regulation of substrates CX3CL1 and CCL2.

Figure 5

HUVECs were treated with siRNA for over 72 h. (A) qPCR was performed for the indicated genes. (B) Specific QC activity in conditioned medium was measured by HPLC and normalized to protein concentration. The activity of basal medium without cells is presented as a horizontal line. (C) Specific QC/isoQC activity in cell extracts was measured by HPLC and normalized to protein concentration. (D) CCL2 was measured via hCCL2 ELISA. (E) ICAM1/CD54 was quantified via immunoassay (MILLIPLEX MAP). Data from six independent experiments are presented as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001).