Skip to main content
. 2017 Aug 24;37(4):BSR20170712. doi: 10.1042/BSR20170712

Figure 7. Induction of mRNA and protein expression in HCASMCs by CX3CL1: pGlu1-residue of CX3CL1 is important for induced expression of CX3CL1, CCL2 and ICAM1/CD54.

Figure 7

HCASMCs were serum-starved for 24 h prior to treatment with Gln1-CX3CL1 or pGlu1-CX3CL1 for the indicated concentrations and times. qPCR was performed for the indicated genes (A) CX3CL1; (B) CCL2; (C) ICAM1/CD54. Data from five independent experiments (cells from two different donors) are expressed as fold-change over untreated. CCL2 levels were quantified with a specific hCCL2 ELISA 4 h (D) and 24 h (E) after treatment. Data from three independent experiments are shown as mean ± S.D. (*P<0.05, **P<0.01, ***P<0.001).