Figure 8. Induction of mRNA and protein expression in HUVECs by CX3CL1: N-terminal pGlu1-residue of CX3CL1 is important for induction of CX3CL1, CCL2 and ICAM1/CD54 expression.

HUVECs were treated with Gln1-CX3CL1 or pGlu1-CX3CL1 (300 ng/ml) for 6 and 24 h. (A–C) Results of qPCR are shown relative to the basal control levels. CCL2 concentrations in supernatant were quantified with a specific hCCL2 ELISA 6 h (D) and 24 h (E) after treatment. Data from four independent experiments (triplicates) are shown as mean ± S.D.