Figure 1.

Carrier-mediated Uptake of [³H]Ergothioneine, [³H]Carnitine, [¹⁴C]αMG and [³H]Gly-Sar by Mice Kidney Slices. Uptake of (A) [³H]ergothioneine (1.0 μM), (B) [³H]carnitine (1.3 nM) and (C) [¹⁴C]αMG (2.5 μM) was performed in the presence or in the absence of Na⁺ into mice kidney slices at pH 7.4 and 37 °C for 1, 3, 5, 10, 15 and 20 min. Closed and open circles represent each substrate uptake in the presence or the absence of Na⁺, respectively. (D) [³H]Gly-Sar (36 nM) was performed in the presence or in the absence of Gly-Leu (5 mM) into mice kidney slices at pH 7.4 and 37 °C for 1, 3, 5, 10, 15 and 20 min. Closed and open circles represent [³H]Gly-Sar uptake in the presence or the absence of Gly-Leu, respectively. Each result represents the mean ± S.E.M (n = 3).* Indicates a significant difference from the uptake in the absence of Na⁺ or Gly-Leu at each time point (p < 0.05) by Student’s t-test.