Figure 3.

Inhibitory Effects of Various Compounds on Uptake by Mice Kidney Slices and Transporter-expressing HEK293 Cells. (A) Uptake of [³H]ergothioneine (1.0 μM) into mice kidney slices was performed in the presence or the absence of the indicated compounds at pH 7.4 and 37 °C for 3 min. Each bar represents the mean ± S.E.M. (n = 3 or 4) after subtraction of the uptake of [³H]ergothioneine in the absence of Na⁺. (B) Uptake of [³H]ergothioneine (1.0 μM) into Octn1-expressing HEK293 cells was performed in the presence or the absence of the indicated compounds at pH 7.4 and 37 °C for 15 sec. Each bar represents the mean ± S.E.M. (n = 3) after subtraction of the uptake of [³H]ergothioneine in the mock cells. (C) Effect of pH or Pept inhibitors on [³H]Gly-Sar uptake in mice kidney slices was performed at pH 7.4 or 6.0, and 37 °C for 5 min. Each bar represents the mean ± S.E.M. (n = 3). (D) Effect of dapagliflozin combination with D-galactose (30 mM) on [¹⁴C]αMG uptake into kidney mice slices was performed at pH 7.4 and 37 °C for 5 min. Each bar represents the mean ± S.E.M. (n = 3) after subtraction of the uptake of [¹⁴C]αMG in the absence of Na⁺. (E) Effect of dapagliflozin combination with D-glucose (0, 1 and 10 mM) on [¹⁴C]αMG uptake was performed at pH 7.4 and 37 °C for 5 min. Each bar represents the mean ± S.E.M. (n = 3) after subtraction of the uptake of [¹⁴C]αMG in the absence of Na⁺. * indicates a significant difference from the control (p < 0.05) by dunnett test.