Figure 1.

Recombinase-mediated DNA inversion and its application to the implementation of a logic gate. (a) Schematic illustration of the irreversible inversion of DNA sequences using serine recombinases. (b) Implementation of an AND gate using recombinases. The right-turn arrow represents a promoter; the red and blue triangles are the targeting sites of recombinases Bxb1 and phiC31, respectively; the letter T’s flanked by the targeting sites are transcription terminators; the green box represents the gene encoding the green fluorescent protein.