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. 2017 Oct 9;7:12876. doi: 10.1038/s41598-017-13232-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of olaparib on cisplatin mediated PARP1 hyperactivation. (A) SiHa and ME180 cells were treated with cisplatin and/or olaparib for 48 hrs and expression of PARP1 and PAR was determined by western blot. Olaparib exerts strong anti-PARP activity in cisplatin treated CC cells.The full size blots corresponding to the cropped blot images are given in Supplementary Figure S12. (B) Bar graph displaying relative PARylation normalized to GAPDH in both cell lines. Error bars display the S.D. from the mean of 3 independent experiments. **p = 0.0095. (C) Representative immunofluorescence image showing nuclear co-localization of PAR polymers with γH2A.X foci. 24 hr cisplatin treated cells show higher PAR polymers signals compare to untreated and signal was completely suppressed after olaparib treatment or PARP1 siRNA transfection. (D) Bar graph displaying quantification of γH2A.X⁺ PAR⁻ or γH2A.X⁺ PAR⁺ cell distribution in cisplatin and/or olaparib treated along with PARP1 silenced cells treated with cisplatin.