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. 2017 Oct 9;7:12874. doi: 10.1038/s41598-017-12497-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PUF60 on HBV production. (a) Particle-associated HBV DNA in culture supernatants of cells transfected with pUC-HB-Ce and pcDNA-F-PUF60 or empty vector (EV) was quantitatively measured at days 2 and 5 pt. (b) After 12 h of transfection with pcDNA-F-PUF60 or EV, HepG2-hNTCP-C4 cells were infected with HBV and cultured for 5 days, and total cellular RNA was analyzed by RT-qPCR to determine levels of 3.5 kb RNA (left) and spRNAs (right). (a) and (b) Values shown represent means ± SD obtained from three independent samples. Statistical differences compared with the control (EV) are shown. *p < 0.05, Student’s t test.