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. 2017 Oct 10;12(10):e0183950. doi: 10.1371/journal.pone.0183950

Fig 1. In vitro characterizations of the contribution of the HPI to motility in UPEC strain NU14.

Fig 1

(A) Deletions of both irp2 and ybtA resulted in the reduction of the motility diameter on LB soft agar plates (0.3%). Motility was restored in complemented strains. qPCR for flagellar genes during motility in wild type strain NU14 (B) and in HPI mutants (C). Data were normalized to 16S rRNA. Bacteria rotating in LB at 37°C were used as the calibrator for (B) and wild type strain NU14 during motility served as the calibrator for (C). (D) Detection of flagellin expression using H7 antiserum. Lane 1, NU14 wild type; lane 2, NU14 Δirp2; lane 3, NU14 ΔybtA. (E) The impact of yersiniabactin production on motility in yersiniabactin-negative strains. CFT073 ybt+ and DH5α ybt+ displayed increased motility compared to the respective wild type strains. All experiments were performed in triplicates and repeated at least three times. Error bars represent standard deviations. *p<0.05, **p<0.01, ***p<0.001.