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. 2017 Oct 10;12(10):e0183950. doi: 10.1371/journal.pone.0183950

Fig 2. The loss of flagellar expression does not influence the activity of the HPI under iron deplete condition.

Fig 2

(A) qPCR for flagellar genes during motility in strains NU14 wild type and flagellar mutant NU14 ΔfliI. Deletion of fliI resulted in the loss of flagellar gene expression of fliC, fliD and flgK. Data were normalized to 16S rRNA. Bacteria shaken in LB at 37°C were used as the calibrator. (B) Detection of flagellin expression using H7 antiserum. Flagellar mutant NU14 ΔfliI showed no expression of flagellin. (C) Mutant strain NU14 ΔfliI was non-motile on LB soft agar plates. (D) qPCR for HPI genes of NU14 wild type and NU14 ΔfliI under iron deplete condition. Transcription rates for ybtA, irp2 and fyuA of the flagellar mutant NU14 ΔfliI displayed no difference relative to the wild type strain NU14 under iron restriction. (E) Detection of FyuA expression in NBD medium. No difference in FyuA expression could be detected between the wild type strain and the flagellar mutant. NU14 ΔfliI showed no expression of FyuA under iron rich conditions in LB medium.