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. 2017 Oct 10;12(10):e0183950. doi: 10.1371/journal.pone.0183950

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© 2017 Magistro et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Construction of transcriptional lacZ reporter fusions with different fragments of the ybtA promoter region. (B) Influence of the YbtA expression on the ybtA promoter activity under iron depletion. The additional expression of YbtA showed a decrease in miller units compared to the empty vector control in NU14 wild type (***p<0.001), but increased significantly the reporter signal in the HPI-negative strains DH5α and NU14 Δhpi, respectively (***p<0.001). (C) The reporter activity of NU14 wild type strain carrying p3RS cultivated in NBD medium, LB medium and during motility on LB soft agar plates. Elevated miller units could be detected only under iron scarcity and during motility (***p<0.001). The reporter activity for different fragments of the ybtA promoter region under iron restriction and during motility in UPEC strain NU14 (D) and K12 E. coli DH5α (E). In both backgrounds the highest promoter activity was only detected with construct p3RS for both growth in NBD medium and during motility on LB soft agar plates.