| irp2 |
irp2.KO.for |
CAGCAGTTACATGAAGAGAGCAACCTGATCCAGGCCGGCCTGGAGTGTAGGCTGGAGCTGCTTC |
| irp2 |
irp2.KO.rev |
GTTTGAGTTCACGGAGTAATTCGACGCCGGACCAGTGGCGATGCTCATATGAATATCCTCCTTA |
| ybtA |
ybtA.KO.for |
ATGATGGAGTCACCGCAAACGCAATCTGAAATCTCTATTCACCAGTTGGTGGTCGGTGTAGGCTGGAGCTGCTTC |
| ybtA |
ybtA.KO.rev |
CATCCCGCGTTTAAAGGTCGAAGGAGTTACGCCAAACTGTTTCTGGAAGGCGGCACATATGAATATCCTCCTTA |
| irp2 |
irp2.KO.for |
CAGCAGTTACATGAAGAGAGCAACCTGATCCAGGCCGGCCTGGAGTGTAGGCTGGAGCTGCTTC |
| irp2 |
irp2.KO.rev |
GTTTGAGTTCACGGAGTAATTCGACGCCGGACCAGTGGCGATGCTCATATGAATATCCTCCTTA |
| fliI |
fliI.KO.for |
AGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAGTGTAGGCTGGAGCTGCTTC |
| fliI |
fliI.KO.rev |
GATCTTTCAGGGTCGCCAGCGCACCATGTTCTGCCATCTGCCGTTATCTCCTGGGCATATGAATATCCTCCTTA |
| Primers and probes for TaqMan-PCR |
| genes |
primers and probes |
sequences (5’-3’) |
| 16SrRNA |
16SrRNA.for |
TTGACGTTACCCGCAGAAGAA |
| 16SrRNA |
16SrRNA.rev |
GCTTGCACCCTCCGTATTACC |
| 16SrRNA |
16SrRNA.probe |
FAM-CGGCTAACTCCGTGCCAGCAGC-TAMRA |
| fyuA |
fyuA.for |
ACACCCGCGAGAAGTTAAATTC |
| fyuA |
fyuA.rev |
AGCGGTGGTATAGCCGGTACT |
| fyuA |
fyuA.probe |
FAM-CCTACGACATGCCGACAATGCCTTATTTAA-TAMRA |
| ybtA |
ybtA.for |
GTTGCCTCTCCTGCCACTTC |
| ybtA |
ybtA.rev |
ATCAGCCAGCAGCAGATCCT |
| ybtA |
ybtA.probe |
FAM-ACCCGATGGAACGCCAGAAACTG-TAMRA |
| Irp2 |
Irp2.for |
TGGGTGCCGGGTGAATTA |
| Irp2 |
Irp2.rev |
CGTCCGGGAGCGTCAA |
| Irp2 |
Irp2.probe |
FAM-ATTTCAACGATCCCCTGCGTAGC-TAMRA |
| fliC |
fliC.for |
CAGGCGATTGCTAACCGTTT |
| fliC |
fliC.rev |
ATACCATCGTTGGCGTTACGT |
| fliC |
fliC.probe |
FAM-TTCTAACATTAAAGGCCTGACTCAGGC-TAMRA |
| fliD |
fliD.for |
GCGTAAGCGCAAGCATCATT |
| fliD |
fliD.rev |
GCCGGTGTCATTTGATGTGA |
| fliD |
fliD.probe |
FAM-ACGTGGGTAACGGTGAATATCGTCT-TAMRA |
| flgK |
flgK.for |
GGGAATAAAACCGCGACGTT |
| flgK |
flgK.rev |
GGAAAGCTGCGTCACCACAT |
| flgK |
flgK.probe |
FAM-AAAACCAGTAGCGCCACGCAAGGT-TAMRA |
| Primers for cloning |
| genes |
primers |
sequences (5’-3’) |
| ybtA |
PybtA.KpnI.for |
TAGCAGggtaccCTGAATTTCCTGATGAATTT |
| ybtA |
ybtA.PstI.rev |
TGCATCctgcagGGCCTCTGTCAGGGAGGAGT |
| Irp2 |
Pirp2.KpnI.for |
TAGCACggtaccCCGGGGTCGCGCCCCCCTAA |
| Irp2 |
Irp2.PstI.rev |
TCACTActgcagCTATATCCGCCGCTGACGAC |
| 3RS |
PybtA.KpnI.for |
TAGCAGggtaccCTGAATTTCCTGATGAATTT |
| 3RS |
PybtA.PstI.rev |
TCAGCActgcagGACCTGGTTATCTCCCTGTG |
| 2RS+ |
PybtA.2RS+.KpnI.for |
TAGCAGggtaccTGGCGTTCTGAGAATTAATG |
| 2RS+ |
PybtA.PstI.rev |
TCAGCActgcagGACCTGGTTATCTCCCTGTG |
| 2RS |
PybtA.2RS.KpnI.for |
TAGCAGggtaccAACTCATCTACCCCATTCGG |
| 2RS |
PybtA.PstI.rev |
TCAGCActgcagGACCTGGTTATCTCCCTGTG |
| 1RS |
PybtA.1RS.KpnI.for |
TAGCAGggtaccTATACCCGCATTGGTCTAAG |
| 1RS |
PybtA.PstI.rev |
TCAGCActgcagGACCTGGTTATCTCCCTGTG |
