Figure 3.

The OAMB's functional site for appetitive learning is the mushroom body αβ and γ neurons. A, Behavioral analysis of hypomorphic oamb alleles. oamb²³⁶ with normal mushroom body OAMB expression displayed performance comparable to that of the ry control; however, oamb¹²⁶ with significantly reduced mushroom body OAMB showed severe deficit in acquisition of appetitive memory like oamb²⁸⁶- and oamb⁵⁸⁴-null mutants (n = 6–18). B, Transgenic OAMB expression induced by the mushroom body driver C747-GAL4 or MB247-GAL4 during development and adulthood fully rescued the oamb's learning phenotype (n = 6–17). C, Normal appetitive learning in oamb mutants was reinstated by temporarily restored OAMB-K3 or OAMB-AS or both in the adult mushroom body αβ and γ neurons. The oamb mutants carrying MB247-GAL4 and tubP-GAL80^ts along with UAS-OAMB-K3 or UAS-OAMB-AS or both, upon mild heat treatment before training (dark gray columns), showed learning performance comparable to that of Canton-S (n = 6 for each genotype). The same genotypes without heat treatment (mid-gray columns) or the oamb²⁸⁶ (^§) and oamb²⁸⁶/oamb⁹⁶ (^§§) carrying only MB247-GAL4 and tub-GAL80^ts subjected to the same temperature shift (light gray columns), however, showed poor learning similar to oamb²⁸⁶. II and III in the genotype table denote the chromosomes containing the transgene. NS, Not significant; *p < 0.05; ***p < 0.001, Tukey–Kramer.