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. 2017 Oct 10;12(10):e0186056. doi: 10.1371/journal.pone.0186056

Fig 2. CRISPR/Cas9-mediated one-step generation of BLG KO goats.

Fig 2

(A) Top panel: PCR products of the target region of BLG from newborn goats micro-injected with Cas9 and single sgRNA. Bottom panel: Results of T7E1 assay of products shown in the top panel. #B1-1 to #B1-8 were injected with sg1, and #B1-9 to #B1-15 were injected with sg2. (B) Top panel: PCR products of the target region of BLG from newborn goats micro-injected with Cas9 and dual sgRNAs. Bottom panel: Results of T7E1 assay of products shown in the top panel. #B2-1 to #B2-4 were injected with sg2+sg3 (25 ng/μL/sgRNA), and #B2-5 to #B2-11 were injected with sg2+sg3 (50 ng/μL/sgRNA). (C) Sequencing results of BLG-modified kids injected with different concentrations. WT, wild-type. PM, point mutation.