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. 2017 Oct 10;12(10):e0186317. doi: 10.1371/journal.pone.0186317

Fig 4. Tyk2 affects early responses to IL-10.

Fig 4

Bone marrow-derived macrophages and dendritic cells from Tyk2-/- mice were investigated in more detail for their signaling. Phosphorylation of tyrosine 705 (Y705) of STAT3 by IL-10 (0, 1, 10 and 100 ng/ml) was analysed in wild-type and Tyk2-/- macrophages and dendritic cells by western blot (A and B respectively). Relative up-regulation of SOCS3 mRNA expression by IL-10 was analysed by quantitative PCR in both macrophages and dendritic cells (C). Fold induction of SOCS3 expression was calculated using the 2ΔCt method using HPRT as a reference gene. Macrophages and dendritic cells from wild-type and Tyk2-/- transgenic mice were pre-treated with IL-10 and were stimulated with 100 ng/ml LPS and the inhibition of TNF-α expression was determined at 2 and 24 hours (D). Asterisk(s) indicate significant differences as determined by a Welch’s t-test (*P<0.05; **P<0.01).