Fig 3. Promotional poster titled “Health Center Preventing Chagas”.

The workflow and poster were developed through participatory activities with Health Center personnel to promote screening for early detection of congenital Chagas disease at the HC. The workflow shows two parallel arrows for the same newborn heel prick blood sample. The blood sample for buffy coat separation in capillary tube is taken simultaneously with the blood sample preserved in nucleic acid preservation cards (FTA) for PCR analysis. The arrow connecting the PCR result with the capillary result indicates confirmation of the microscopy, to optimize the parasitological screening method at the Health Center. Children born to positive mothers, but that are not screened within two weeks of birth, should be brought to the Health Center at ten months for antibody rapid test only.