Table 3. Effect of secondary sugar-feeding on male body and seminal fluid marking.

Diet	Male Body Marked	Male Body Not Marked	Seminal Fluid Marked	Seminal Fluid Not Marked
No additional sugar-feeding	60	1	44	9
Additional sugar-feeding	61	2	47	5

Number of males Ae. aegypti with detectable body and seminal marking 96 h after feeding on rhodamine B (0.4%) for 4 days, with or without additional fluid intake during secondary sugar-feeding (50% honey, no rhodamine B). Assessment of seminal fluid marking was determined by the presence or absence of rhodamine B in the bursa and/or spermathecae of females allowed to mate with an individual male.