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. 2017 Sep 25;11(9):e0005962. doi: 10.1371/journal.pntd.0005962

Fig 3. Immuno-localization and functional evaluation of host immunoglobulins purified from protein extracts of Taenia solium cysts.

Fig 3

A) Immuno-localization of host IgG in cysts obtained from the central nervous system and skeletal muscle of pigs. B) Immunoglobulins present in total protein extracts of cysts were purified using a Sepharose 4B column coupled with Protein G. C). The purity of the bound IgG was evaluated by SDS-PAGE and western blotting using an anti-pig IgG coupled to HRP. D) The antibody activity of purified IgG was evaluated by ELISA. Samples of T. solium vesicular fluid and insoluble fraction were used to coat 96 well microtiter plates. Afterwards, the purified IgG was incubated overnight at 4°C under slow agitation. Then, the antigen-antibody reaction was developed using a colorimetric method. E) Recognition of cysts proteins by the purified IgG through western blotting. Samples of T. solium vesicular fluid and insoluble fraction were resolved through SDS-PAGE and then transferred onto a nitrocellulose membrane. The purified IgG from cysts protein extracts was used as primary antibody (for comparison sera from cysticercotic pigs was also included) and an anti-pig IgG coupled to HRP was used as secondary antibody. Lower right panel shows the reaction with the secondary antibody alone as control.