Figure 3.

PMCA1 immunocytochemistry in the Purkinje neurons. At PD11, control rats (ctr) show intense labelling on the cell membrane of Purkinje cells; the cytoplasm is weakly positive while strong immunoreactivity is present in the molecular layer, in the Purkinje cell dendrite branches. After, cisPt and PtAcacDMS decreased labelling on the cell membrane and in the cytoplasm are observed, as well as in the molecular layer. At PD17, controls show a weak immunoreactivity vs. PD11; changes are observed after both platinum compounds treatment. Histograms show the OD values and the significance of differences is reported (** p < 0.01; *** p < 0.001). egl: External granular layer; ml: Molecular layer; igl: Internal granular layer; Pl: Purkinje cell layer. Scale bar: 50 μm