Figure 4. Infertility caused by SUN5 mutations could be overcome by ICSI.

(A) Representative images and (B) genotypes of the Sun5-null ICSI offspring. (C) Representative images and (D) genotypes of the WT ICSI offspring. (E) Pedigree of family 1 with inherited SUN5 mutations, and the healthy baby of the infertility patient after ICSI. The individuals with a single star were Sanger sequenced. (F) Sequences of the SUN5 mutation sites of the representative individuals from each generation of family 1. (G) Pedigree of family 8 with inherited SUN5 mutations, and the health baby of the infertility patient after ICSI. The individuals with a single star were Sanger sequenced. (H) Sequences of the SUN5 mutation sites of the representative individuals from each generation of family 8.

Figure 4—figure supplement 1. Development of WT and Sun5-null ICSI offspring.

(A) Body weight of WT and Sun5^−/− ICSI offspring, statistical analysis was performed using at least three individuals. (B) Testis weight of adult WT and Sun5^−/− ICSI offspring, statistical analysis was performed using at least three individuals. Data represent mean ±SEM.