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. 2017 Oct 10;6:e27529. doi: 10.7554/eLife.27529

Figure 4. Overexpression of OsSAMS1 enhances RDV infection whereas knockout of OsSAMS1 reduces RDV infection.

(A) Symptoms of the mock-inoculated WT or RDV-infected WT (non-transgenic) plants and OsSAMS1 OX/KO transgenic plants; images were taken at 4 wpi. Scale bars = 10 cm (upper panel) and 5 cm (lower panel). (B) Detection of RDV S2, S8, and S11 genomic segments in the mock-inoculated WT or RDV-infected WT (non-transgenic) plants and in OsSAMS1 OX/KO transgenic plants by northern blot. The blots were hybridized with radiolabeled riboprobes specific for each RNA segment. rRNAs were stained with ethidium bromide and served as loading controls. Tissues were collected at 4 wpi. (C) Detection of RDV P2, P8, and Pns11 proteins in the mock-inoculated WT or RDV-infected WT (non-transgenic) plants and in OsSAMS1 OX/KO transgenic plants by western blot. Actin was probed and served as a loading control. Tissues were collected at 4 wpi. (D) The incidences of infection, which were determined by visual assessment of disease symptoms at 0–8 wpi of 30 individual plants for each case. Means and standard deviations were obtained from three independent experiments.

Figure 4.

Figure 4—figure supplement 1. Overexpression of OsSAMS1 enhances RDV infection while downregulation of OsSAMS1 reduces RDV infection.

Figure 4—figure supplement 1.

(A) Symptoms of mock or RDV-infected WT plants as well as OsSAMS1 OX/RNAi transgenic plants, pictures were taken at 4 wpi. Scale bars, 10 cm (upper panel) and 5 cm (lower panel). (B) Detection of RDV genomic S2, S8, S11 in mock or RDV-infected WT plants as well as in OsSAMS1 OX/RNAi transgenic plants by northern blot. The blots were hybridized with radiolabeled riboprobes specific for each RNA segment. rRNAs were stained with ethidium bromide and served as loading controls. Tissues were collected at 4 wpi. (C) Detection of RDV protein P2, P8, Pns11 in mock or RDV-infected WT plants as well as in OsSAMS1 OX/RNAi transgenic plants by western blot. Actin was probed and served as a loading control. Tissues were collected at 4 wpi. (D) The incidences of infection, which were determined by visual assessment of disease symptoms at 0–8 wpi of 30 individual plants for each case. Means and standard deviations were obtained from three independent experiments.